Title: Validation of a Real-time PCR On-Site Quantification Method for MON810 Maize
Authors: LANGRELL StephenALLNUT TheodoreLAVAL ValerieBERTHEAU YvesPLA MariaPAPAZOVA NinaDAVID Lee
Citation: FOOD ANALYTICAL METHODS vol. 4 no. 3 p. 313-318
Publisher: SPRINGER
Publication Year: 2011
JRC Publication N°: JRC57766
ISSN: 1936-9751
URI: http://www.springerlink.com/content/d214w0t438h77271/
http://publications.jrc.ec.europa.eu/repository/handle/JRC57766
DOI: 10.1007/s12161-010-9167-z
Type: Articles in Journals
Abstract: A rapid on-, or near-site, quantitative method for use as a pre-harvest predictive decision tool for adventitious genetically modified (GM) presence (AGMP) has been developed. Based on a laboratory-based protocol for real-time (RT) quantification of the MON810 GM event in maize kernels, the duplex RT polymerase chain reaction (PCR) method was constructed around the portable Cepheid SmartCyclerII platform, requiring only modest support infrastructure for field application. Pre-validation through an international ring trial showed good compliance with minimum assay performance requirements as defined by the European Network of GMO Laboratories (RSDr = 18.5%; RSDR = 32.8; Bias = 26.7%).
JRC Institute:Institute for Prospective Technological Studies

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