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|Title:||Optimization of chemically defined cell culture media - Replacing Fetal Bovine Serum in mammalian in vitro methods|
|Authors:||VAN DER VALK Jan; DE SMET K.; FEX Svenningsen; HONEGGER Paul; KNUDSEN L.; LINDL T.; NORABERG J.; PRICE Anna; SCARINO M.l.; GSTRAUNTHALER G.; BRUNNER D.|
|Citation:||TOXICOLOGY IN VITRO vol. 24 no. 4 p. 1053-1063|
|Publisher:||PERGAMON-ELSEVIER SCIENCE LTD|
|Type:||Articles in periodicals and books|
|Abstract:||Quality assurance is becoming increasingly important. Good laboratory practice (GLP) and Good manufacturing practice (GMP) are good examples of established standards and often a prerequisite for studies within a regulatory context and for manufacturing processes. In the biomedical field there is a strive towards and increasing reliance on the use of in vitro methods: cell and tissue culture. In vitro methods are generally fast, cheap, reproducible and decrease the reliance on animal studies. Good Cell Culture Practice (GCCP) is an attempt to become a standard for in vitro methods. Culturing cells in a chemically defined medium is part of the GCCP, thereby decreasing the reliance on animal serum, with undefined components, as supplement. Defined media supplements are commercially available for some cell types, but information on the formulation is often limited and these can therefore not be regarded as defined. The development of defined media is difficult and often takes place in isolation. A workshop was organised in 2009 in Copenhagen to discuss strategies to improve the development of serum-free defined media. In this report a strategy is discussed to develop serum-free defined culture media and cell adaptation process. Furthermore, recommendations are proposed to improve information exchange on newly developed media.|
|JRC Directorate:||Institute for Health and Consumer Protection Historical Collection|
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