Title: Preparation of VtiO2 labelled nanoparticles for biokinetics studies
Authors: GIBSON PeterKOZEMPEL JANABBAS KamelSIMONELLI FedericaCYDZIK IzabelaHOLZWARTH UweKREYLING WolfgangSEMMLER-BEHNKE ManuelaWENK A.DAWSON KennethRAMIREZ-GARCIA S.
Citation: CHEMICKE LISTY vol. 104 no. 14 (Special Issue) p. s214-s215
Publisher: CHEMICKE LISTY
Publication Year: 2010
JRC N°: JRC59055
ISSN: 0009-2770
URI: http://www.chemicke-listy.cz/docs/full/2010_14_s212-s221.pdf
http://publications.jrc.ec.europa.eu/repository/handle/JRC59055
Type: Contributions to Conferences
Abstract: Engineered nanoparticles are of current interest in many applications (e.g. industry, medicine, energy). As they are produced in ever higher quantities, the need to assess their biokinetics and toxicity becomes more and more important. The aim of this study was to prepare stable, radiolabelled bioavailable TiO2 nanoparticles to assess their in vivo biokinetics. The preparation of [V-48]TiO2 nanoparticles by direct proton irradiation is described. Irradiations were performed on the Scanditronix MC-40 cyclotron at JRC Ispra, Italy with a 5 µA proton beam of 23.5 MeV energy. After 16 hours irradiation, a commercial sample of TiO2 (ST-01, Ishihara Corp. Japan) yielded approx. 40 MBq of V-48 activity at EOB. Half of the irradiated material was processed two weeks after EOB to size-select and purify the labelled nanoparticles from free V-48 by centrifugation and filtration. This yielded 855 kBq of final suspension of fine [V-48]TiO2 labeled nanoparticles (Z average = 105 nm) without free V-48. In vivo results in a rat model indicated good V-48 label stability with less than 1% of the administered dose eliminated by renal excretion, compared to ionic V-48 with approximately 50% renal clearance.
JRC Institute:Institute for Health and Consumer Protection

Files in This Item:
There are no files associated with this item.


Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.