Title: Feasibility of a Capillary LC/ESI-Q-TOF MS Method for the Detection of Milk Allergens in an Incurred Model Food Matrix
Citation: ANALYTICAL METHODS vol. 2 no. 7 p. 967-972
Publication Year: 2010
JRC N°: JRC59082
ISSN: 1759-9660
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC59082
DOI: 10.1039/c0ay00151a
Type: Articles in periodicals and books
Abstract: The capability of a capillary LC-Q-TOF mass spectrometer system as a qualitative tool for the identification and confirmation of milk allergens in thermally processed food was investigated. Milk powder incurred cookies were produced in-house and chosen as the model food matrix. To unequivocally assess the presence of milk allergens, samples testing positive to ELISA were analysed by a capillary LC-Q-TOF MS/MS method in order to identify specific peptides that can be used as markers for milk allergens. Results show that a-S1 casein was the protein identified with the highest score (in 100 mg g-1 milk powder incurred cookies) and its identity was confirmed by detection of the peptides m/z 692.86 and 634.34 and their specific MS/MS ions providing a fingerprint for a-S1 casein. Besides that, other milk proteins were highlighted by performing database searching. The proteomic MS-based method employing a capillary LC-Q-TOF system proved to be a valuable tool to carry out qualitative and confirmative analysis to trace contamination of milk allergens in processed food matrices.
JRC Directorate:Health, Consumers and Reference Materials

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