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|Title:||Proteomic analysis of the marine diatom Thalassiosira pseudonana upon exposure to benzo(a)pyrene|
|Authors:||NEGRÃO DE CARVALHO RAQUEL; LETTIERI Teresa|
|Citation:||BMC GENOMICS vol. 12 no. 159|
|Publisher:||BIOMED CENTRAL LTD|
|Type:||Articles in Journals|
|Abstract:||Abstract Background The polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants ubiquitously distributed. They are generated by incomplete combustion of organic materials such as wood or fossil fuels. Due to their carcinogenic, mutagenic effects and to their wide distribution in the environment, these pollutants pose many concerns to researchers and regulators. In our laboratories we investigated the effect of Benzo(a)pyrene (BaP) exposure in the marine diatom Thalassiosira pseudonana, which has become an important model organism in aquatic toxicology studies. Results In order to investigate the mechanism of action of PAHs, we exposed the diatoms for 24 h to 36.45 µg/L of B(a)P which inhibits the growth by about 30 % and analysed the relative protein expression profile by a quantitative proteomics approach based on iTRAQ labels. The proteomics profile analysis showed that around 10 % of the identified proteins were regulated and one fourth of them confirmed the gene expression changes seen by DNA microarray. Particularly interesting was the down regulation of the Silicon transporter 1 (SIT1), an enzyme that is responsible for the uptake of silica from the media into the cell of diatoms. Regulation of SIT1 upon BaP treatment was also confirmed at the gene expression level. Conclusions The potential use of the regulated proteins found in this study as early indicators of environmental exposure to PAHs is discussed. In particular, SIT1 is considered a promising biomarker and SIT1 expression changes were confirmed also when the diatoms were exposed to field samples, e.g. marine surface sediments contaminated by PAHs.|
|JRC Institute:||Institute for Environment and Sustainability|
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