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|Title:||A Printed Nanoliter-Scale Bacterial Sensor Array|
|Authors:||MELAMED S.; CERIOTTI Laura; WEIGEL W.; ROSSI Francois; COLPO Pascal; BELKIN S.|
|Citation:||LAB ON A CHIP vol. 11 no. 1 p. 139-146|
|Publisher:||ROYAL SOC CHEMISTRY|
|Type:||Articles in periodicals and books|
|Abstract:||The last decade has witnessed a significant increase in interest in whole-cell biosensors for diverse applications, as well as a rapid and continuous expansion of array technologies. The combination of these two disciplines has yielded the notion of whole-cell array biosensors. We present a potential manifestation of this idea by describing the printing of a whole-cell bacterial bioreporters array. Exploiting natural bacterial tendency to adhere to positively charged a-biotic surfaces, we describe immobilization and patterning of bacterial ¿spots¿ in the nanoliter volume range by a non-contact robotic arrayer. We show that the printed Escherichia coli-based sensor bacteria are immobilized on the surface, and retain their viability and biosensing activity for at least 2 months when kept at 4 oC. Immobilization efficiency was improved by manipulating the bacterial genetics (overproducing curli protein), the growth and printing media (osmotic stress and osmoprotectants) and by a chemical modification of the inanimate surface (self-assembled layers of 3-aminopropyltrimethoxysilane). We suggest that the methodology presented herein may be applicable to the manufacturing of whole-cell sensor arrays for diverse high throughput applications.|
|JRC Institute:||Institute for Health and Consumer Protection|
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