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|Title:||Development of a Recombinant Fab-Fragment Based Electrochemical Immunosensor for Deoxynivalenol Detection in Food Samples|
|Authors:||ROMANAZZO Daniela; RICCI Francesco; VOLPE Giulia; ELLIOTT Christopher T.; VESCO Silvia; KROEGER-NEGOITA KATY; MOSCONE Danila; STROKA Joerg; VAN EGMOND Hans; VEHNIAINEN Markus; PALLESCHI Giuseppe|
|Citation:||BIOSENSORS & BIOELECTRONICS vol. 25 no. 12 p. 2615-2621|
|Publisher:||ELSEVIER ADVANCED TECHNOLOGY|
|Type:||Articles in Journals|
|Abstract:||A reliable and cost-effective electrochemical method for the detection of deoxynivalenol (DON) in cereals and cereal-based food samples based on the use of a novel anti-DON Fab-fragment is presented. The analytical system employed. Enzyme-Linked-Immunomagnetic-Electrochemical (ELIME) assay, is based on the use of immunomagnetic beads (IMBs) coupled with eight magnetized screen-printed electrodes (8-mScPEs) as electrochemical transducers. Using standard solutions of DON, a working range between 100 and 4500 ng/ml was obtained with an EC50 of 380 ng/ml. The ELIME assay was employed to evaluate the cross-reactivity of the Fab-fragment towards different trichothecenes revealing a good selectivity towards DON over other trichothecenes with exception of 3-Ac-DON. The sensor was then applied to cereals and cereal-based food samples (wheat, breakfast cereal and baby-food) and a wide range of sample treatment procedures was tested. Within-laboratory precision (9-24% repeatability for breakfast cereals and 10-33% for baby-food) and recovery data (82-110% for breakfast cereals and 97-108% for baby-food) were calculated by analysing blank breakfast cereals and baby-foods fortified with DON, demonstrating that the proposed method has the capability for use as a screening assay for DON in such products.|
|JRC Institute:||Institute for Reference Materials and Measurements|
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