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|Title:||Inflammation and reactive oxygen/nitrogen species in glial/neuronal cultures|
|Authors:||NEHER Jonas J.; BROWN Guy C.; KINSNER-OVASKAINEN Agnieszka; PRICE Anna|
|Type:||Articles in books|
|Abstract:||Inflammation contributes to a wide variety of brain pathologies. In this chapter methods are described for using microglia and astrocytes in culture to investigate inflammatory processes and inflammatory neurodegeneration, including the use of neuronal/glial co-cultures and transwells. Methods for detecting and characterising inflammatory activation in culture, including changes in microglial phenotype,microglial phagocytosis and expression of pro-inflammatory cytokines . The protocols for how to determine cellular production of superoxide, hydrogen peroxide, peroxynitrite and nitric oxide in culture are described.|
|JRC Institute:||Institute for Health and Consumer Protection|
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