Title: Inflammation and reactive oxygen/nitrogen species in glial/neuronal cultures
Publisher: Springer
Publication Year: 2011
JRC N°: JRC59783
ISBN: 978-1-61779-076-8
ISSN: 0893-2336
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC59783
DOI: 10.1007/978-1-61779-077-5_16
Type: Articles in periodicals and books
Abstract: Inflammation contributes to a wide variety of brain pathologies. In this chapter methods are described for using microglia and astrocytes in culture to investigate inflammatory processes and inflammatory neurodegeneration, including the use of neuronal/glial co-cultures and transwells. Methods for detecting and characterising inflammatory activation in culture, including changes in microglial phenotype,microglial phagocytosis and expression of pro-inflammatory cytokines . The protocols for how to determine cellular production of superoxide, hydrogen peroxide, peroxynitrite and nitric oxide in culture are described.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

Files in This Item:
There are no files associated with this item.

Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.