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|Title:||Three-dimensional automated nanoparticle tracking using Mie scattering in an optical microscope|
|Authors:||GINESTE Jean-Michel; MACKO Peter; PATTERSON Eann; WHELAN Maurice|
|Citation:||JOURNAL OF MICROSCOPY-OXFORD vol. 243 no. 2 p. 172-178|
|JRC Publication N°:||JRC60981|
|Type:||Articles in Journals|
|Abstract:||The forward scattering of light in a conventional inverted optical microscope by nanoparticles ranging in diameter from 10 to 50nm has been used to automatically and quantitatively identify and track their location in three-dimensions with a temporal resolution of 200ms and a spatial precision of about 5nm. The microscope objective was oscillated along the light path using a piezo actuator and images acquired with the condenser aperture closed to a minimum to enhance the effects of diffraction. Data processing in the time and spatial domains allowed the location of particles to be obtained automatically so that the technique has applications both in the processing of nanoparticles and in their use in a variety of fields including nanobiotechnology, pharmaceuticals and food processing where a simple optical microscope maybe preferred for a variety of reasons.|
|JRC Institute:||Institute for Health and Consumer Protection|
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