Title: Optimization and Prevalidation of the in Vitro ER CALUX Method to Test Estrogenic and Antiestrogenic Activity of Compounds
Authors: VAN DER BURG BartWINTER RoosWEIMER MarcBECKMANNS PascaleSUZUKI GoGIJSBERG LindaJONAS ArjenVAN DER LINDEN SanderWITTERS HildaAARTS JacLEGLER JulietteKOOP-SCHNEIDER AnnetteBREMER Susanne
Citation: REPRODUCTIVE TOXICOLOGY vol. 30 no. 1 p. 73-80
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Publication Year: 2010
JRC N°: JRC61239
ISSN: 0890-6238
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC61239
DOI: 10.1016/j.reprotox.2010.04.007
Type: Articles in Journals
Abstract: Estrogenicity of chemicals has received significant attention and is linked to endocrine-disrupting activities. However, there is a paucity of validated methods to assess estrogenicity in vitro.We have established a robust method to test estrogenic and antiestrogenic activity of compounds in vitro, as an alternative to using animal models such as the uterotrophic assay. To this end we optimized protocols to be used in combination with CALUX reporter gene assays and carried out an in house prevalidation, followed by two rounds of tests to establish transferability. Problems in the initial test with transferability were solved by isolation of a novel cell clone of the ER CALUX line with greatly improved stability and luciferase levels. This cell line proved to be a very suitable and reliable predictor of estrogenicity of chemicals and was able to readily rank a range of chemicals on the basis of their EC50 values.
JRC Institute:Institute for Health and Consumer Protection

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