Title: Selective inhibition of B lymphocytes in TBTC-treated human bone marrow long-term culture
Authors: CARFI MariaBOWE GerardPIETERS RaymondGRIBALDO Laura
Citation: TOXICOLOGY vol. 276 no. 1 p. 33-40
Publisher: ELSEVIER IRELAND LTD
Publication Year: 2010
JRC N°: JRC62244
ISSN: 0300-483X
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC62244
DOI: 10.1016/j.tox.2010.06.012
Type: Articles in Journals
Abstract: Tributyltin chloride (TBTC) is well known for its immunotoxic effect, in particular towards immature thymocytes. TBTC is also known to induce adipocyte differentiation in primary human bone marrow cultures, which is reflected in the decrease in a number of adipocyte-derived cytokines, chemokines and the adipocyte-linked hormone leptin. Since adipocytes influence haematopoiesis and lymphopoiesis for instance by these cytokines and hormones, we here investigated whether TBTC has an effect on specific lymphocyte subsets in human bone marrow primary cultures. FACS analysis showed a reduction of CD19/CD22-positive B cells by TBTC, both in the presence or absence of cytokines. The treatment did not cause a toxic effect on mature CD3+CD4+ and CD3+CD8+ T cells, suggesting selective TBTC toxicity on B lymphocytes in the presently used in vitro system.
JRC Institute:Institute for Health and Consumer Protection

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