Title: 56Co-labelled radioactive Fe3O4 nanoparticles for in vitro uptake studies on Balb/3T3 and Caco-2 cell lines
Authors: MARMORATO PatrickSIMONELLI FedericaABBAS KamelKOZEMPEL JANHOLZWARTH UweFRANCHINI FabioPONTI JessicaROSSI FrancoisGIBSON Peter
Citation: JOURNAL OF NANOPARTICLE RESEARCH vol. 13 no. 12 p. 6707-6716
Publisher: SPRINGER
Publication Year: 2011
JRC Publication N°: JRC62501
ISSN: 1388-0764
URI: http://www.springerlink.com/content/x585371558107780/
http://publications.jrc.ec.europa.eu/repository/handle/JRC62501
DOI: 10.1007/s11051-011-0577-7
Type: Articles in Journals
Abstract: Magnetite nanoparticles (Fe3O4 NPs) are one of the manufactured nanomaterials increasingly used in healthcare for different medical applications from diagnosis to therapy. In the present work Fe3O4 NPs have been irradiated with a proton beam in order to produce 56Co as radiolabel and the possibility has been investigated to use nuclear techniques for the quantification of these Fe3O4 NPs in biological systems. In particular, the size distribution and surface charge of the NPs have been characterized before and after the irradiation process in order to verify that these essential properties were preserved during the irradiation. The radiation emitted from the radiolabels has then been used to quantify the cellular uptake of these NPs in in-vitro studies. For biological applications two cell lines were selected: immortalized mouse fibroblast cell line (Balb/3T3) and human epithelial colorectal adenocarcinoma cell line (Caco-2). At each step of this study, the cell uptake has been quantified by activity measurements of the 56Co. These measurements were performed with the high resolution ¿-ray spectrometry. This study showed that under well established irradiation conditions, Fe3O4 NPs do not undergo significant structural modifications and the obtained results are in line with uptake studies using the same non activated nanomaterial. Therefore the radiolabelling method can be useful to study uptake due to low-level exposure where a higher sensitivity is required.
JRC Institute:Institute for Health and Consumer Protection

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