Title: Report on the Verification of the Performance of MON 89034, 1507, MON 88017 and 59122 Event-specific Methods on the Maize Event MON 89034 x 1507 x MON 88017 x 59122 Using Real-Time PCR. Validation Report and Protocols
Authors: LUQUE PEREZ EncarnacionBEVILACQUA A.LARCHER SaraPINSKI GregorMAZZARA MarcoVAN DEN EEDE Guy
Publisher: Publications Office of the European Union
Publication Year: 2010
JRC N°: JRC62814
ISBN: 978-92-79-19082-7
ISSN: 1018-5593
Other Identifiers: EUR 24706 EN
OPOCE LB-NA-24706-EN-C
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC62814
DOI: 10.2788/31795
Type: EUR - Scientific and Technical Research Reports
Abstract: The European Union Reference Laboratory for GM Food and Feed (EURL-GMFF), established by Regulation (EC) No 1829/2003, has carried out a verification study to assess the performance of four quantitative event-specific methods on the maize event MON 89034 x 1507 x MON 88017 x 59122 (unique identifier MON-89Ø34-3 x DAS-Ø15Ø7-1 x MON-88Ø17-3 x DAS-59122-7) which combines the MON 89034, 1507, MON 88017 and 59122 transformation events. The four methods have been validated individually on single-trait events, to detect and quantify each event in maize samples. This study was conducted according to internationally accepted guidelines (1, 2). In accordance to Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and to Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Monsanto and Dow AgroSciences provided the detection methods and the control samples: genomic DNA from homogenised seeds of MON 89034 x 1507 x MON 88017 x 59122 maize (258-4CC, 258-4JJ, 258-4B, 258-4N) and from homogenised seeds of conventional maize (10001262-V). The EURL-GMFF prepared the verification samples (calibration samples and blind samples at different GM percentages). The results of the verification study were evaluated with reference to ENGL method performance requirements (http://gmo-crl.jrc.ec.europa.eu/guidancedocs.htm) and to the validation results on the individual parental events (http://gmo-crl.jrc.ec.europa.eu/statusofdoss.htm). The results of this EURL-GMFF verification study are publicly available at http://gmo-crl.jrc.ec.europa.eu/.
JRC Institute:Institute for Health and Consumer Protection

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