Title: Current Perspectives and Recommendations for the Development of Mass Spectrometry Methods for the Determination of Allergens in Foods
Authors: JOHNSON Phil E.BAUMGARTNER S.ALDICK ThomasBESSANT ConradGIOSAFATTO ValeriaHEICK JuliaMAMONE GianfrancoO'Connor G.Poms R.POPPING BertREUTER AndreasULBERTH FranzWATSON AndrewMONACI LindaMILLS Clare
Citation: JOURNAL OF AOAC INTERNATIONAL vol. 94 no. 4 p. 1026-1033
Publisher: AOAC INT
Publication Year: 2011
JRC N°: JRC65103
ISSN: 1060-3271
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC65103
Type: Articles in Journals
Abstract: Allergen detection and quantification is an essential part of allergen management as practiced by food manufacturers. Recently, protein MS methods (in particular, multiple reaction monitoring experiments) have begun to be adopted by the allergen detection community to provide an alternative technique to ELISA and PCR methods. MS analysis of proteins in foods provides additional challenges to the analyst, both in terms of experimental design and methodology: (1) choice of analyte, including multiplexing to simultaneously detect several biologically relevant molecules able to trigger allergic reactions; (2) choice of processing stable peptide markers for different target analytes that should be placed in publicly available databases; (3) markers allowing quantification (e.g., through standard addition or isotopically labeled peptide standards); (4) optimization of protease digestion protocols to ensure reproducible and robust method development; and (5) effective validation of methods and harmonization of results through the use of naturally incurred reference materials spanning several types of food matrix.
JRC Institute:Institute for Reference Materials and Measurements

Files in This Item:
There are no files associated with this item.


Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.