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|Title:||Current challenges in detecting food allergens by shotgun and targeted proteomic approaches: A case study on traces of peanut allergens in baked cookies|
|Authors:||PEDRESCHI ROMINA; NORGAARD Jorgen; MAQUET Alain|
|Citation:||NUTRIENTS vol. 4 no. 2 p. 132-150|
|JRC Publication N°:||JRC67360|
|Type:||Articles in Journals|
|Abstract:||There is a need for selective and sensitive methods to detect the presence of food allergens at trace levels in highly processed food products. In this work, a combination of non-targeted and targeted proteomics approaches are used to illustrate the difficulties encountered in the detection of the major peanut allergens Ara h 1, Ara h 2 and Ara h 3 from a representative processed food matrix. Shotgun proteomics was employed for selection of the proteotypic peptides for targeted approaches via selective reaction monitoring. Peanut presence through detection of the proteotypic Ara h 3/4 peptides AHVQVVDSNGNR (m/z 432.5, 3+) and SPDIYNPQAGSLK (m/z 695.4, 2+) was confirmed and the developed method was able to detect peanut presence at trace levels (≥10 μg peanut g−1 matrix) in baked cookies.|
|JRC Institute:||Institute for Reference Materials and Measurements|
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