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|Title:||Artificial Defocus for Displaying Markers in Microscopy Z-Stacks|
|Authors:||GIUSTI Alessandro; TADDEI PIERLUIGI; CORANI Giorgio; MAGLI Cristina; GIANAROLI Luca; GAMBARDELLA Luca|
|Citation:||IEEE TRANSACTIONS ON VISUALIZATION AND COMPUTER GRAPHICS vol. 17 no. 12 p. 1757 - 1764|
|Publisher:||IEEE COMPUTER SOC|
|DOI:||1757 - 1764|
|Type:||Articles in periodicals and books|
|Abstract:||As microscopes have a very shallow depth of field, Z-stacks (i.e. sets of images shot at different focal planes) are often acquired to fully capture a thick sample. Such stacks are viewed by users by navigating them through the mouse wheel. We propose a new technique of visualizing 3D point, line or area markers in such focus stacks, by displaying them with a depth-dependent defocus, simulating the microscope's optics; this leverages on the microscopists' ability to continuously twiddle focus, while implicitly performing a shape-from-focus reconstruction of the 3D structure of the sample. User studies confirm that the approach is effective, and can complement more traditional techniques such as color-based cues. We provide two implementations, one of which computes defocus in real time on the GPU, and examples of their application.|
|JRC Directorate:||Nuclear Safety and Security|
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