Title: Artificial Defocus for Displaying Markers in Microscopy Z-Stacks
Authors: GIUSTI AlessandroTADDEI PIERLUIGICORANI GiorgioMAGLI CristinaGIANAROLI LucaGAMBARDELLA Luca
Citation: IEEE TRANSACTIONS ON VISUALIZATION AND COMPUTER GRAPHICS vol. 17 no. 12 p. 1757 - 1764
Publisher: IEEE COMPUTER SOC
Publication Year: 2011
JRC N°: JRC67533
ISSN: 1077-2626
URI: http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=6064938
http://publications.jrc.ec.europa.eu/repository/handle/JRC67533
DOI: 1757 - 1764
Type: Articles in Journals
Abstract: As microscopes have a very shallow depth of field, Z-stacks (i.e. sets of images shot at different focal planes) are often acquired to fully capture a thick sample. Such stacks are viewed by users by navigating them through the mouse wheel. We propose a new technique of visualizing 3D point, line or area markers in such focus stacks, by displaying them with a depth-dependent defocus, simulating the microscope's optics; this leverages on the microscopists' ability to continuously twiddle focus, while implicitly performing a shape-from-focus reconstruction of the 3D structure of the sample. User studies confirm that the approach is effective, and can complement more traditional techniques such as color-based cues. We provide two implementations, one of which computes defocus in real time on the GPU, and examples of their application.
JRC Institute:Institute for Transuranium Elements

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