Title: Detection of airborne genetically modified maize pollen by real-time PCR
Authors: FOLLONI SilviaKAGKLI DAFNIRAJCEVIC B.CASTANHEIRA GUIMARÃES NcVAN DROOGENBROECK BVALICENTE FhVAN DEN EEDE GuyVAN DEN BULCKE MARC
Citation: MOLECULAR ECOLOGY RESOURCES vol. 12 no. 5 p. 810-821
Publisher: WILEY-BLACKWELL
Publication Year: 2012
JRC Publication N°: JRC67794
ISSN: 1755-098X
URI: http://onlinelibrary.wiley.com/doi/10.1111/men.2012.12.issue-5/issuetoc
http://publications.jrc.ec.europa.eu/repository/handle/JRC67794
DOI: 10.1111/j.1755-0998.2012.03168.x
Type: Articles in Journals
Abstract: The cultivation of genetically modified (GM) crops has raised numerous concerns in the European Union and other parts of the world about their environmental and economic impact. Especially outcrossing of genetically modified organisms (GMO) was from the beginning a critical issue as airborne pollen has been considered an important way of GMO dispersal. Here, we investigate the use of airborne pollen sampling combined with microscopic analysis and molecular PCR analysis as an approach to monitor GMmaize cultivations in a specific area. Field trial experiments in the European Union and South America demonstrated the applicability of the approach under different climate conditions, in rural and semiurban environment, even at very low levels of airborne pollen. The study documents in detail the sampling of GMpollen, sample DNA extraction and real-time PCR analysis. Our results suggest that this ‘GM pollen monitoring by bioaerosol sampling and PCR screening’ approach might represent an useful aid in the surveillance of GM-free areas, centres of origin and natural reserves.
JRC Institute:Institute for Health and Consumer Protection

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