Title: Event-specific Method for the Quantification of Maize MIR162 by Real-time PCR
Authors: CHARLES DELOBEL ChrystèleMAZZARA MarcoBEVILACQUA A.VAN DEN EEDE Guy
Publisher: Publications Office of the European Union
Publication Year: 2011
JRC N°: JRC68155
ISBN: 978-92-79-22645-8
ISSN: 1831-9424
Other Identifiers: EUR 25149 EN
OPOCE LB-NA-25149-EN-N
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC68155
DOI: 10.2788/44161
Type: EUR - Scientific and Technical Research Reports
Abstract: The European Union Reference Laboratory for Genetically Modified Food and Feed (EURL-GMFF), established by Regulation (EC) No 1829/2003, in collaboration with the European Network of GMO Laboratories (ENGL), has carried out a collaborative study to assess the performance of a quantitative event-specific method to detect and quantify the MIR162 transformation event (unique identifier SYN-IR162-4) in maize DNA. The collaborative study was conducted according to internationally accepted guidelines (1, 2). In accordance to Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and with Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Syngenta Seeds S.A.S. provided the detection method and the control samples (genomic DNA extracted from homogenised seeds containing the transformation event and from conventional homogenised seeds). The EURL-GMFF prepared the validation samples (calibration samples and blind samples at unknown GM percentage [DNA/DNA]). The collaborative study involved twelve laboratories from nine European countries. The results of the international collaborative study met the ENGL performance requirements. The method is therefore considered applicable to the control samples provided, in accordance with the requirements of Annex I-2.C.2 to Commission Regulation (EC) No 641/2004. The results of the collaborative study are made publicly available at http://gmo-crl.jrc.ec.europa.eu/.
JRC Institute:Institute for Health and Consumer Protection

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