Title: Evaluation of plasmid and genomic DNA calibrants used for the quantification of genetically modified organisms
Authors: CAPRIOARA-BUDA MihaelaMEYER WIBKEJEYNOV BoyanCORBISIER PhilippeTRAPMANN StefanieEMONS Hendrik
Citation: ANALYTICAL AND BIOANALYTICAL CHEMISTRY vol. 404 no. 1 p. 29-42
Publisher: SPRINGER HEIDELBERG
Publication Year: 2012
JRC N°: JRC69642
ISSN: 1618-2642
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC69642
DOI: 10.1007/s00216-012-6104-6
Type: Articles in Journals
Abstract: The reliable quantification of genetically modified organisms (GMOs) by real-time PCR requires, besides thoroughly validated quantitative detection methods, sustainable calibration systems. The latter establishes the anchor points for the measured value and the measurement unit, respectively. In this paper, the suitability of two types of DNA calibrants, i.e. plasmid DNA and genomic DNA extracted from plant leaves, for the certification of the GMO content in reference materials as copy number ratio between two targeted DNA sequences was investigated. The paper outlines, that the most suitable approach to establish a sustainable traceability chain is to fix a reference system based on plasmid DNA.
JRC Institute:Institute for Reference Materials and Measurements

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