Title: The certification of different mass fractions of AV43-6-G7 in potato tuber powder: ERM®-BF431a, ERM®-BF431b, ERM®-BF431c, ERM®-BF431d and ERM®-BF431e
Authors: TRAPMANN StefanieCONNEELY PatrickCORBISIER PhilippeKORTEKAAS Anna MariaMAZOUA StephaneSEGHERS JOHN
Publisher: Publications Office of the European Union
Publication Year: 2012
JRC N°: JRC70337
ISBN: 978-92-79-24171-0
ISSN: 1831-9424
Other Identifiers: EUR 25301 EN
OPOCE LA-NA-25301-EN-N
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC70337
DOI: 10.2787/60535
Type: EUR - Scientific and Technical Research Reports
Abstract: This report describes the production of a set of Certified Reference Materials (CRMs) ERM-BF431a, b, c, d and e, matrix materials certified for their AV43-6-G7 mass fractions. The material has been produced following ISO Guide 34:2009. Genetically modified (GM) tubers of the potato event AV43-6-G7 and of a non-GM potato variety were dried and ground to obtain GM and non-GM potato powders. Beside these two pure materials gravimetric mixtures of non-GM and GM potato powder were prepared by dry-mixing. Between unit-heterogeneity has been quantified and stability during dispatch and storage have been assessed in accordance with ISO Guide 35:2006. The certified value was obtained from the gravimetric preparations, taking into account the purity of the base materials and their water mass fraction. The certified values were confirmed by event-specific real-time PCR as independent verification method (measurements within the scope of accreditation to ISO/IEC 17025:2005). Uncertainties of the certified values were calculated in compliance with the Guide to the Expression of Uncertainty in Measurement (GUM) and include uncertainties related to possible heterogeneity, instability, and characterisation. The materials are intended for the calibration or quality control of methods. As any reference material, they can also be used for control charts or validation studies. The CRMs are available in glass vials containing at least 1 g of dried potato tuber powder and closed under argon atmosphere. The minimum amount of sample to be used is 200 mg. The CRM has been accepted as European Reference Material (ERM®) after peer evaluation by the partners of the European Reference Materials consortium.
JRC Directorate:Health, Consumers and Reference Materials

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