Title: Size-dependent toxicity and cell interaction mechanisms of gold nanoparticles on mouse fibroblasts
Authors: CORADEGHINI RosellaGIORIA SabrinaPASCUAL GARCIA CESARNATIVO PaolaFRANCHINI FabioGILLILAND DouglasPONTI JessicaROSSI Francois
Citation: TOXICOLOGY LETTERS vol. 217 no. 3 p. 205-216
Publisher: ELSEVIER IRELAND LTD
Publication Year: 2013
JRC N°: JRC71598
ISSN: 0378-4274
URI: http://www.sciencedirect.com/science/article/pii/S0378427412014026
http://publications.jrc.ec.europa.eu/repository/handle/JRC71598
DOI: 10.1016/j.toxlet.2012.11.022
Type: Articles in periodicals and books
Abstract: This work presents an in vitro toxicological study of gold nanoparticles (AuNPs), which investigates cellular uptake and possible mechanisms of nanoparticles internalization. After a detail characterization of the physico-chemical properties of AuNPs 5 and 15 nm citrate stabilized, cytotoxicity was assessed in vitro on Balb/3T3 cells. No toxicity was detected by Trypan Blue, while by CFE toxicity was found for 5 nm AuNPs at concentration ≥ 50 µM after 72 h of exposure. Using Transmission Electron Microscopy (TEM) we verified that NPs were taken-up by cells, and the extent of the uptake was quantified by Inductively Coupled Mass Spectroscopy (ICP-MS). The involvement of clathrin and caveolin in the mechanism of nanoparticles cell internalization was investigated using immunocytochemistry and western blot, and no changes in the expression levels of these proteins were found. Following exposure to AuNPs, the cell footprints became narrow and contracted and disruption of the cytoskeleton structure was evident.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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