Title: Interlaboratory comparison study of the Colony Forming Efficiency assay for assessing cytotoxicity of nanomaterials
Authors: PONTI JessicaKINSNER-OVASKAINEN AgnieszkaNORLEN H.ALTMEYER SandrineANDREOLI CristinaBOGNI AlessiaCHEVILLARD SylvieDE ANGELIS IsabellaCHUNG Seung-TaeEOM IgchunFUJITA KatsuhideGILLILAND DouglasGROLLINO Maria GiuseppaGULUMIAN MaryHIRSCH CordulaICHIRAKU KayoIGARASHI TakuyaJEONG JayoungJO EunhyeKIM Dang-YoungKAISER Jean-PierreLAGACHE DorisLA SPINA RITALEE Jong KwonLEE JieunLOVERA AnnalisaMÄDER-ALTHAUS XeniaNESSLANY FabriceOJEA JIMENEZ ISAACPACCHIEROTTI FrancescaPIANELLA FRANCESCAPAGET VincentKIM Tae RimROSZAK JoannaROSENKRANZ PHILIPPSIMAR SophieSTĘPNIK MaciejVETTEN MelissaSONG Nam WoongYANG Jun-YoungROSSI Francois
Editors: KINSNER-OVASKAINEN Agnieszka
PONTI Jessica
Publisher: Publications Office of the European Union
Publication Year: 2014
JRC N°: JRC92910
ISBN: 978-92-79-44677-1
ISSN: 1831-9424
Other Identifiers: EUR 27009
OP LB-NA-27009-EN-N
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC92910
DOI: 10.2788/406937
Type: EUR - Scientific and Technical Research Reports
Abstract: Nanotechnology has gained importance in the past years as it provides opportunities for industrial growth and innovation. However, the increasing use of manufactured nanomaterials (NMs) in a number of commercial applications and consumer products raises also safety concerns and questions regarding potential unintended risks to humans and the environment. Since several years the European Commission’s Joint Research Centre (JRC) is putting effort in the development, optimisation and harmonisation of in vitro test methods suitable for screening and hazard assessment of NMs. Work is done in collaboration with international partners, in particular the Organisation for Economic Co-operation and Development (OECD). This report presents the results from an interlaboratory comparison study of the in vitro Colony Forming Efficiency (CFE) cytotoxicity assay performed in the frame of OECD's Working Party of Manufactured Nanomaterials (WPMN). Twelve laboratories from European Commission, France, Italy, Japan, Poland, Republic of Korea, South Africa and Switzerland participated in the study coordinated by JRC. The results show that the CFE assay is a suitable and robust in vitro method to assess cytotoxicity of NMs. The assay protocol is well defined and is easily and reliably transferable to other laboratories. The results obtained show good intra and interlaboratory reproducibility of the assay for both the positive control and the tested nanomaterials. In conclusion the CFE assay can be recommended as a building block of an in vitro testing battery for NMs toxicity assessment. It could be used as a first choice method to define dose-effect relationships for other in vitro assays.
JRC Institute:Institute for Health and Consumer Protection



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