@article{JRC42890, address = {AMSTERDAM (NETHERLANDS)}, year = {2008}, author = {Monaci L and Van Hengel A}, abstract = {The availability of accurate and sensitive detection methods for food allergens is crucial for the food industry to ensure the correct labelling of their products in order to protect allergic consumers. For this purpose a method using solid-phase-extraction and liquid chromatography coupled to mass spectrometry was developed to detect traces of three allergenic cow milk proteins (lactalbumin, lactoglobulin A and B) in mixed fruit juice samples. Different sample pre-treatments were compared and the best recoveries were obtained with a method employing a SPE cartridge. Recoveries ranging from 68% to 79% were achieved for 5 and 20 ppm tested and the limit of detection was set at 1 ppm. Both full scan mode and multiple ion monitoring acquisition modes were investigated and compared. The method was utilized to analyse 15 mixed-fruit juices collected from the market and was found to be capable of positively identifying all three milk proteins. The developed method enables the unambiguous determination of allergenic whey proteins in mixed-fruit juices and can assist in the protection of milk allergic individuals. }, title = {Development of a Method for the Quantification of Whey Allergen Traces in Mixed-Fruit Juices Based on Liquid Chromatography with Mass Spectrometric Detection}, type = {}, url = {https://dx.doi.org/10.1016/j.chroma.2008.03.041}, volume = {1192}, number = {}, journal = {JOURNAL OF CHROMATOGRAPHY A}, pages = {113-120}, issn = {0021-9673}, publisher = {ELSEVIER SCIENCE BV}, doi = {10.1016/j.chroma.2008.03.041} }