The Impact of Superficially Porous Particles and New Stationary-Phase Chemistries on the LC–MS Determination of Mycotoxins in Food and Feed

Superficially porous particles (also known as fused-core, porous-shell, core-shell, solid-core) with their favourable chromatographic properties were a great advance for liquid chromatography. Analytical LC columns packed with those particles allow for much faster separations even with standard LC equipment rated at a maximum pressure of 400 bar. This is exemplified by a LC-MS method of analysis for four mycotoxins, spanning log P values from -0.7 to 3.6, with an analysis time of just over 8 min and excellent performance. Another issue is the separation of closely related mycotoxins, like 3- and 15-Acetyldeoxynivalenol. With the common C18 chemistries they coelute and identification and quantification can only be achieved through differing MS/MS signals. Now, with the newer pentafluorophenyl chemistries these two mycotoxins can be separated by LC and MS quantification of them has become much more precise.

BREIDBACH Andreas; 

2017-02-03

LC GC

JRC100285