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dc.contributor.authorFORCELLA Matildeen_GB
dc.contributor.authorCALLEGARO Giuliaen_GB
dc.contributor.authorMELCHIORETTO Pasqualeen_GB
dc.contributor.authorGRIBALDO Lauraen_GB
dc.contributor.authorFRATTINI M.en_GB
dc.contributor.authorSTEFANINI F.m.en_GB
dc.contributor.authorFUSI Paolaen_GB
dc.contributor.authorURANI Chiaraen_GB
dc.date.accessioned2016-10-27T00:11:44Z-
dc.date.available2016-10-25en_GB
dc.date.available2016-10-27T00:11:44Z-
dc.date.created2016-10-24en_GB
dc.date.issued2016en_GB
dc.date.submitted2016-01-27en_GB
dc.identifier.citationTOXICOLOGY IN VITRO vol. 36 p. 71-80en_GB
dc.identifier.issn0887-2333en_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC100330-
dc.description.abstractThe in vitro Cell Transformation Assay (CTA) is a powerful tool for mechanistic studies of carcinogenesis. The endpoint is the classification of transformed colonies (foci) bymeans of standard morphological features. To increase throughput and reliability of CTAs, one of the suggested follow-up activities is to exploit the comprehension of the mechanisms underlying cell transformation. To this end, we have performed CTAs testing CdCl2, a widespread environmental contaminant classified as a human carcinogen with the underlying mechanisms of action not completely understood. We have isolated and re-seeded the cells at the end (6 weeks) of in vitro CTAs to further identify the biochemical pathways underlying the transformed phenotype of foci. Morphological evaluations and proliferative assays confirmed the loss of contact-inhibition and the higher proliferative rate of transformed clones. The biochemical analysis of EGFR pathway revealed that, despite the same initial carcinogenic stimulus (1 μM CdCl2 for 24 h), transformed clones are characterized by the activation of two different molecular pathways: proliferation (Erk activation) or survival (Akt activation). Our preliminary results on molecular characterization of cell clones from different foci could be exploited for CTAs improvement, supporting the comprehension of the in vivo process and complementing the morphological evaluation of foci.en_GB
dc.description.sponsorshipJRC.F.3-Chemicals Safety and Alternative Methodsen_GB
dc.format.mediumOnlineen_GB
dc.languageENGen_GB
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDen_GB
dc.relation.ispartofseriesJRC100330en_GB
dc.titleCadmium-transformed cells in the in vitro Cell Transformation Assay reveal different proliferative behaviors and activated pathwaysen_GB
dc.typeArticles in periodicals and booksen_GB
dc.identifier.doi10.1016/j.tiv.2016.07.006en_GB
JRC Directorate:Health, Consumers and Reference Materials

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