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|Title:||Label-Free Proteomic Analysis of Wheat Gluten Proteins and Their Immunoreactivity to ELISA Antibodies|
|Authors:||MARTINEZ ESTESO MARIA JOSÉ; BROHEE MARCEL; NORGAARD JORGEN; O'CONNOR GAVIN|
|Citation:||Cereal Chemistry p. 1-7|
|Type:||Articles in periodicals and books|
|Abstract:||ELISA (enzyme linked immunosorbent assay) methodology is currently the mainstay for gluten quantification. However, the lack of comparable measurements among commercial kits has caused a great deal of concern. Here, we studied the immunoreactivity of five commercial ELISA kits to wheat gluten fractionated by reversed phase high-performance liquid chromatography (RP-HPLC) and identified the proteins and peptides in the resulting fractions by mass spectrometry to understand the extent by which these may be contributing to the lack of comparability.|
|JRC Directorate:||Health, Consumers and Reference Materials|
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