Title: Label-Free Proteomic Analysis of Wheat Gluten Proteins and Their Immunoreactivity to ELISA Antibodies
Authors: MARTINEZ ESTESO MARIA JOSÉBROHEE MARCELNORGAARD JORGENO'CONNOR GAVIN
Citation: Cereal Chemistry p. 1-7
Publisher: AACC INTERNATIONAL
Publication Year: 2017
JRC N°: JRC100860
ISSN: 0009-0352
URI: https://doi.org/10.1094/CCHEM-11-16-0266-R
http://publications.jrc.ec.europa.eu/repository/handle/JRC100860
DOI: 10.1094/CCHEM-11-16-0266-R
Type: Articles in periodicals and books
Abstract: ELISA (enzyme linked immunosorbent assay) methodology is currently the mainstay for gluten quantification. However, the lack of comparable measurements among commercial kits has caused a great deal of concern. Here, we studied the immunoreactivity of five commercial ELISA kits to wheat gluten fractionated by reversed phase high-performance liquid chromatography (RP-HPLC) and identified the proteins and peptides in the resulting fractions by mass spectrometry to understand the extent by which these may be contributing to the lack of comparability.
JRC Directorate:Health, Consumers and Reference Materials

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