Title: Novel nuclear barcode regions for the identification of flatfish species
Authors: PARACCHINI VALENTINAPETRILLO MAUROLIEVENS ANTOONPUERTAS GALLARDO ANTONIOMARTINSOHN JANNHOFHERR JOHANNMAQUET ALAINBARBOSA DA SILVA ANA PAULAKAGKLI DAFNI MARIAQUERCI MADDALENAPATAK DENNSTEDT ALEXANDREANGERS ALEXANDRE
Citation: FOOD CONTROL vol. 79 p. 297-308
Publisher: ELSEVIER SCI LTD
Publication Year: 2017
JRC N°: JRC103129
ISSN: 0956-7135
URI: http://dx.doi.org/10.1016/j.foodcont.2017.04.009
http://publications.jrc.ec.europa.eu/repository/handle/JRC103129
DOI: 10.1016/j.foodcont.2017.04.009
Type: Articles in periodicals and books
Abstract: The development of an efficient seafood traceability framework is crucial for the management of sustainable fisheries and the monitoring of potential substitution fraud across the food chain. Recent studies have shown the potential of DNA barcoding methods in this framework, with most of the efforts focusing on using mitochondrial targets such as the cytochrome oxidase 1 and cytochrome b genes. In this article, we show the identification of novel targets in the nuclear genome, and their associated primers, to be used for the efficient identification of flatfishes of the Pleuronectidae family. In addition, different in silico methods are described to generate a dataset of barcode reference sequences from the ever-growing wealth of publicly available sequence information, replacing, where possible, labour-intensive laboratory work. The short amplicon lengths render the analysis of these new barcode target regions ideally suited to next-generation sequencing techniques, allowing haracterisation of multiple fish species in mixed and processed samples. Their location in the nucleus also improves currently used methods by allowing the identification of hybrid individuals.
JRC Directorate:Health, Consumers and Reference Materials

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