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dc.contributor.authorPAINI ALICIAen_GB
dc.contributor.authorMENNECOZZI MILENAen_GB
dc.contributor.authorHORVAT TOMISLAVen_GB
dc.contributor.authorGERLOFF KIRSTEN BRITTAen_GB
dc.contributor.authorPALOSAARI TAINAen_GB
dc.contributor.authorSALA BENITO JOSE'en_GB
dc.contributor.authorWORTH ANDREWen_GB
dc.date.accessioned2017-10-18T00:32:47Z-
dc.date.available2017-10-16en_GB
dc.date.available2017-10-18T00:32:47Z-
dc.date.created2017-10-11en_GB
dc.date.issued2016en_GB
dc.date.submitted2016-10-24en_GB
dc.identifier.citationTOXICOLOGY IN VITRO vol. 45 no. Part 2 p. 233-240en_GB
dc.identifier.issn0887-2333en_GB
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0887233316302144en_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC103486-
dc.description.abstractThe Virtual Cell Based Assay (VCBA) was applied to simulate the long-term (repeat dose) toxic effects of chemicals, including substances in cosmetics and personal care products. The presented model is an extension of the original VCBA for simulation of single exposure and is implemented in a KNIME workflow. This work illustrates the steps taken to simulate the repeated dose effects of two reference compounds, caffeine and amiodarone. Using caffeine, in vitro experimental viability data in single exposure from two human liver cell lines, HepG2 and HepaRG, were measured and used to optimize the VCBA, subsequently repeated exposure simulations were run. Amiodarone was then tested and simulations were performed under repeated exposure conditions in HepaRG. The results show that the VCBA can adequately predict repeated exposure experiments in liver cell lines. The refined VCBA model can be used not only to support the design of long term in vitro experiments but also practical applications in risk assessment. Our model is a step towards the development of in silico predictive approaches to replace, refine, and reduce the in vivo repeated dose systemic toxicity studies in the assessment of human safety.en_GB
dc.description.sponsorshipJRC.F.3-Chemicals Safety and Alternative Methodsen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDen_GB
dc.relation.ispartofseriesJRC103486en_GB
dc.titlePractical use of the Virtual Cell Based Assay: Simulation of repeated exposure experiments in liver cell linesen_GB
dc.typeArticles in periodicals and booksen_GB
dc.identifier.doi10.1016/j.tiv.2016.10.007en_GB
JRC Directorate:Health, Consumers and Reference Materials

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