Title: Proteomics study of silver nanoparticles on Caco-2 cells
Authors: GIORIA SABRINAURBAN LOPEZ PATRICIAHAJDUCH MARTINBARBORO PAOLACABALEIRO DOMINGUEZ NOELIALA SPINA RITACHASSAIGNE HUBERT
Citation: TOXICOLOGY IN VITRO vol. 50 p. 347-372
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Publication Year: 2018
JRC N°: JRC103862
ISSN: 0887-2333
URI: https://www.sciencedirect.com/science/article/pii/S0887233318301188?via%3Dihub
http://publications.jrc.ec.europa.eu/repository/handle/JRC103862
DOI: 10.1016/j.tiv.2018.03.015
Type: Articles in periodicals and books
Abstract: Silver nanoparticles (AgNPs) have been incorporated into several consumer products. While these advances in technology are promising and exciting, the effects of these nanoparticles have not equally been studied. Due to the size, AgNPs can penetrate the body through oral exposure and reach the gastrointestinal tract. The present study was designed as a comparative proteomic analysis of Caco-2 cells, used as an in vitro model of the small intestine, exposed to 30 nm citrate stabilized-silver nanoparticles (AgNPs) for 24 or 72 h. Using two complementary proteomic approaches, 2D gel-based and label-free mass spectrometry, we present insight into the effects of AgNPs at proteins level. Exposure of 1 or 10 µg/mL AgNPs to Caco-2 cells resulted in 56 and 88 altered proteins at 24 h and 72 h respectively, by 2D gel-based technique. Ten of these proteins were found to be common between the two time-points. Using label-free mass spectrometry technique, 291 and 179 altered proteins were found at 24 h and 72 h, of which 24 were in common. Analysis of the proteomes showed several major biological processes altered, from which, cell cycle, cell morphology, cellular function and maintenance were the most affected.
JRC Directorate:Health, Consumers and Reference Materials

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