Multiplex cell microarrays for high-throughput screening

Microarray technology was developed in the early 1990s to measure the transcription levels of thousands of genes in parallel. The basic premise of high-density arraying has since been expanded to create cell microarrays. Cells on chip are powerful experimental tools for high-throughput and multiplex screening of samples or cellular functions. Miniaturization increases assay throughput while reducing both reagent consumption and cell population heterogeneity effect, making these systems attractive for a wide range of assays,from drug discovery to toxicology, stem cell research and therapy. It is usual to functionalize the surface of a substrate to design cell microarrays. One form of cell microarrays, the transfected cell microarray, wherein plasmid DNA or siRNA spotted on the surface of a substrate is reverse-transfected locally into adherent cells, has become a standard tool for parallel cell-based analysis. With the advent of technology, cells can also be directly spotted onto functionalized surfaces using robotic fluid-dispensing devices or printed directly on bio-ink material. We are providing herein an overview of the latest developments in optical cell microarrays allowing high-throughput and high-content analysis.

BERTHUY Ophelie;  MULDUR Sinan;  ROSSI Francois;  COLPO Pascal;  BLUM L.;  MARQUETTE Christophe; 

2016-11-22

ROYAL SOC CHEMISTRY

JRC103894

1473-0197,   

https://publications.jrc.ec.europa.eu/repository/handle/JRC103894,   

10.1039/c6lc00831c,