Title: Can currently available non-animal methods detect pre and prohaptens relevant for skin sensitization?
Authors: Patlewicz GraceCASATI SilviaBASKETTER DavidASTURIOL BOFILL DAVIDROBERTS DavidLEPOITTEVIN Jean PierreWORTH AndrewASCHBERGER KARIN
Citation: REGULATORY TOXICOLOGY AND PHARMACOLOGY p. 1-9
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Publication Year: 2016
JRC N°: JRC104160
ISSN: 0273-2300
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC104160
Type: Articles in periodicals and books
Abstract: Predictive testing to characterize substances for their skin sensitization potential has historically been based on animal tests such as the Local Lymph Node Assay (LLNA). In recent years, regulations in the cosmetics and chemicals sectors have provided strong impetus to develop non-animal alternatives. Three test methods have undergone OECD validation: the direct peptide reactivity assay (DPRA), the KeratinoSens ™and the human Cell Line Activation Test (h-CLAT). Whilst these methods perform relatively well in predicting LLNA results, a concern raised is their ability to predict chemicals that need activation to be sensitizing (pre- or pro-haptens). This current study reviewed an EURL ECVAM dataset of 127 substances for which information was available in the LLNA and three non-animal test methods. Twenty eight of the sensitizers needed to be activated, with the majority being pre-haptens. These were correctly identified by 1 or more of the test methods. Six substances were categorized exclusively as pro-haptens, but were correctly identified by at least one of the cell-based assays. The analysis here showed that skin metabolism was not likely to be amajor consideration for assessing sensitization potential and that sensitizers
JRC Directorate:Health, Consumers and Reference Materials

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