Title: Inter-laboratory studies for the validation of two singleplex (tE9 and pea lectin) and one multiplex (pat/bar) real-time PCR methods for GMO detection
Authors: DEBODE FrédéricHUBER IngridMACARTHUR RoyRISCHITOR PATRICIA ELENAMAZZARA MarcoHERAU V.SEBAH DanielaDOBNIK DavidBROEDERS SylviaROOSENS NancyBUSCH UlrichBERBEN GilbertMORISSET DanyZEL Jana
Citation: FOOD CONTROL vol. 73 p. 452-461
Publisher: ELSEVIER SCI LTD
Publication Year: 2016
JRC N°: JRC104986
ISSN: 0956-7135
URI: http://dx.doi.org/10.1016/j.foodcont.2016.08.037
http://publications.jrc.ec.europa.eu/repository/handle/JRC104986
DOI: 10.1016/j.foodcont.2016.08.037
Type: Articles in periodicals and books
Abstract: This paper presents the inter-laboratory studies for the successful validation of qualitative real-time Polymerase Chain Reaction methods for the detection of Genetically Modified Organisms in food and feed. Two singleplex TaqMan methods were developed for the detection of the tE9 terminator present in several genetically modified events and the detection of the endogenous lectin gene from pea; the tE9 terminator donor organism. In addition, a TaqMan duplex method was validated, which allows the simultaneous detection of the pat and bar elements. The paper explains the different acceptance parameters used during the in-house validation, the method transferability study and finally the inter-laboratory validation, how this validation was conducted and the problems encountered. Statistical methods for the validation of the qualitative methods are also presented. This information can be used by laboratories for the verification of these methods during their implementation and for the validation of new methods.
JRC Directorate:Health, Consumers and Reference Materials

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