Full metadata record
DC FieldValueLanguage
dc.contributor.authorKAEMPFER ANGELAen_GB
dc.contributor.authorURBAN LOPEZ PATRICIAen_GB
dc.contributor.authorGIORIA SABRINAen_GB
dc.contributor.authorKANASE NILESHen_GB
dc.contributor.authorSTONE VICKIen_GB
dc.contributor.authorKINSNER-OVASKAINEN AGNIESZKAen_GB
dc.date.accessioned2017-12-14T01:19:47Z-
dc.date.available2017-12-12en_GB
dc.date.available2017-12-14T01:19:47Z-
dc.date.created2017-12-04en_GB
dc.date.issued2017en_GB
dc.date.submitted2017-03-08en_GB
dc.identifier.citationTOXICOLOGY IN VITRO vol. 45 p. 31-43en_GB
dc.identifier.issn0887-2333en_GB
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0887233317302278?via%3Dihuben_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC106079-
dc.description.abstractThe intestine forms the largest interface between the environment and the human organism. Its integrity and functioning are crucial for the uptake of nutrients while preventing access of harmful antigens. Inflammatory conditions can significantly change the normal functioning of the intestine. In vitro models that adequately reproduce both healthy and inflamed intestinal tissue could provide a useful tool for studying the mechanisms of intestinal inflammation and investigating new therapeutic drugs. We established a co-culture of Caco-2 and PMA-differentiated THP-1 cells that mimics the intestine in healthy and controlled inflamed states. In homoeostatic conditions without stimulation, Caco-2 and THP-1 cells were co-cultured for 48 h without affecting the barrier integrity and with no increase in the release of cytokines, nitric oxide or lactate dehydrogenase. To simulate the inflamed intestine, the Caco-2 barrier was primed with IFN-γ and THP-1 cells were pre-stimulated with LPS and IFN-γ. In these conditions a significant but temporary reduction in barrier integrity was measured, and large concentrations of pro-inflammatory cytokines and cytotoxicity markers detected. With its ability to feature numerous hallmarks of intestinal inflammation the presented co-culture model of epithelial cells and macrophages offers a unique possibility to study exposure effects in relation to the health status of the intestine.en_GB
dc.description.sponsorshipJRC.F.1-Health in Societyen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDen_GB
dc.relation.ispartofseriesJRC106079en_GB
dc.titleDevelopment of an in vitro co-culture model to mimic the human intestine in healthy and diseased state.en_GB
dc.typeArticles in periodicals and booksen_GB
dc.identifier.doi10.1016/j.tiv.2017.08.011en_GB
JRC Directorate:Health, Consumers and Reference Materials

Files in This Item:
There are no files associated with this item.


Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.