Please use this identifier to cite or link to this item:
|Title:||From in vitro to in vivo: Integration of the Virtual Cell Based Assay with Physiologically Based Kinetic modelling|
|Authors:||PAINI ALICIA; SALA BENITO JOSE'; BESSEMS JOSEPH; WORTH ANDREW|
|Citation:||TOXICOLOGY IN VITRO vol. 45 no. part 2 p. 241-248|
|Publisher:||PERGAMON-ELSEVIER SCIENCE LTD|
|Type:||Articles in periodicals and books|
|Abstract:||Physiologically Based Kinetic (PBK) models and the Virtual Cell Based Assay can be linked to form so called Physiologically Based Dynamic (PBD) models. This study illustrates the development and application of a PBK model for prediction of estragole-induced DNA adduct formation and hepatotoxicity in humans. To address the hepatotoxicity, HepaRG cells were used as a surrogate for liver cells, with cell viability being used as the in vitro toxicological endpoint. Information on DNA adduct formation was taken from the literature. Since estragole induced cell damage is not directly caused by the parent compound, but by a reactive metabolite, information on the metabolic pathway was incorporated into the model. In addition, a user-friendly tool was developed by implementing the PBK/D model into a KNIME workflow. This workflow can be used to perform in vitro to in vivo extrapolation and forward as backward dosimetry in support of chemical risk assessment.|
|JRC Directorate:||Health, Consumers and Reference Materials|
Files in This Item:
There are no files associated with this item.
Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.