Title: Ongoing inflammation enhances the toxicity of engineered nanomaterials: Application of an in vitro co-culture model of the healthy and inflamed intestine
Authors: KAEMPFER ANGELAURBAN LOPEZ PATRICIALA SPINA RITAOJEA JIMENEZ ISAACKANASE NILESHSTONE VICKIKINSNER-OVASKAINEN AGNIESZKA
Citation: TOXICOLOGY IN VITRO vol. 63 no. 104738 p. 1-11
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Publication Year: 2020
JRC N°: JRC108975
ISSN: 0887-2333 (online)
URI: https://doi.org/10.1016/j.tiv.2019.104738
https://publications.jrc.ec.europa.eu/repository/handle/JRC108975
DOI: 10.1016/j.tiv.2019.104738
Type: Articles in periodicals and books
Abstract: Chronic inflammatory conditions can negatively impact intestinal barrier function and affect the epithelium's interaction with nano-sized materials. We demonstrate the application of a Caco-2/THP-1 co-culture mimicking the intestine in healthy (i.e. stable) or inflamed state in nanotoxicological research. The co-cultures were exposed to non-toxic concentrations of silver nanoparticles (AgNPs) or silver nitrate (AgNO3) for 24 h. The barrier integrity and cytokine release as well as necrotic and apoptotic cell death were investigated. AgNPs and AgNO3 most strongly affected the inflamed co-culture. Higher concentrations of AgNPs induced a significant increase in barrier integrity in the inflamed but not the stable co-culture. Necrotic and apoptotic cell death was detected in both conditions but were significantly more pronounced in the inflamed condition. The exposure to AgNO3 affected barrier integrity in all experimental set-ups, but caused nuclear condensation only in the Caco-2 monoculture and the inflamed co-culture. AgNPs reduced the release of monocyte chemoattractant protein-1 in the stable model. Clear differences were observed in the effects of AgNPs and AgNO3 in relation to the model's health status. The results suggest an increased vulnerability of the inflamed epithelial barrier towards AgNPs underlining the importance to consider the intestinal health status in the safety assessment of nanomaterials.
JRC Directorate:Health, Consumers and Reference Materials

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