Multi-functionalized nanoparticles are of great interest in biotechnology and biomedicine, especially for diagnostic and therapeutic purposes. However, at the moment the characterization of complex, multi-functional nanoparticles is still challenging and this hampers the development of advanced nanomaterials for biological applications. In this work, we have designed a model system consisting of gold nanoparticles functionalized with two differentially-terminated poly(ethylene oxide) ligands, providing both “stealth” properties and protein-binding capabilities to the nanoparticles. We use a combination of techniques (CLS, DLS, AF4, TEM, Z-potential, SDS-PAGE, and CD to: i) monitor and quantify the ratios of ligand molecules per nanoparticle; ii) determine the effect of coating density on nonspecific protein adsorption; iii) to assess the number and structure of the covalently-bound proteins. This article aims at comparing the complementary outcomes from typical and orthogonal techniques used on nanoparticle characterization by employing a versatile nanoparticle-ligands-biomolecule model system.
OJEA JIMENEZ Isaac;
CAPOMACCIO Robin Bruno;
OSORIO Ines;
MĖHN Dóra;
CECCONE Giacomo;
HUSSAIN Rohanah;
SILIGARDI Giuliano;
COLPO Pascal;
ROSSI Francois;
GILLILAND Douglas;
CALZOLAI Luigi;
2018-07-12
ROYAL SOC CHEMISTRY
JRC109517
2040-3364,
http://pubs.rsc.org/en/Content/ArticleLanding/2018/NR/C8NR00973B#!divAbstract,
https://publications.jrc.ec.europa.eu/repository/handle/JRC109517,
10.1039/c8nr00973b,
| Name | Country | City | Type |
|---|
This document is only visible at the Commission level.
You are not authorized to publish or distribute it outside the European Commission.
This is a public document. You can share this publication.
Datasets
| ID | Title | Public URL |
|---|
Dataset collections
| ID | Acronym | Title | Public URL |
|---|
Scripts / source codes
| Description | Public URL |
|---|
Additional supporting files
| File name | Description | File type |
|---|