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|Title:||Rational design of multi-functional gold nanoparticles with controlled biomolecule adsorption: a multi-method approach for in-depth characterization|
|Authors:||OJEA JIMENEZ ISAAC; CAPOMACCIO ROBIN BRUNO; OSORIO INES; MĖHN DÓRA; CECCONE GIACOMO; HUSSAIN ROHANAH; SILIGARDI GIULIANO; COLPO PASCAL; ROSSI FRANCOIS; GILLILAND DOUGLAS; CALZOLAI LUIGI|
|Citation:||NANOSCALE vol. 10 no. 21 p. 10173-10181|
|Publisher:||ROYAL SOC CHEMISTRY|
|Type:||Articles in periodicals and books|
|Abstract:||Multi-functionalized nanoparticles are of great interest in biotechnology and biomedicine, especially for diagnostic and therapeutic purposes. However, at the moment the characterization of complex, multi-functional nanoparticles is still challenging and this hampers the development of advanced nanomaterials for biological applications. In this work, we have designed a model system consisting of gold nanoparticles functionalized with two differentially-terminated poly(ethylene oxide) ligands, providing both “stealth” properties and protein-binding capabilities to the nanoparticles. We use a combination of techniques (CLS, DLS, AF4, TEM, Z-potential, SDS-PAGE, and CD to: i) monitor and quantify the ratios of ligand molecules per nanoparticle; ii) determine the effect of coating density on nonspecific protein adsorption; iii) to assess the number and structure of the covalently-bound proteins. This article aims at comparing the complementary outcomes from typical and orthogonal techniques used on nanoparticle characterization by employing a versatile nanoparticle-ligands-biomolecule model system.|
|JRC Directorate:||Health, Consumers and Reference Materials|
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