Title: Rational design of multi-functional gold nanoparticles with controlled biomolecule adsorption: a multi-method approach for in-depth characterization
Authors: OJEA JIMENEZ ISAACCAPOMACCIO ROBIN BRUNOOSORIO INESMĖHN DÓRACECCONE GIACOMOHUSSAIN ROHANAHSILIGARDI GIULIANOCOLPO PASCALROSSI FRANCOISGILLILAND DOUGLASCALZOLAI LUIGI
Citation: NANOSCALE vol. 10 no. 21 p. 10173-10181
Publisher: ROYAL SOC CHEMISTRY
Publication Year: 2018
JRC N°: JRC109517
ISSN: 2040-3364
URI: http://pubs.rsc.org/en/Content/ArticleLanding/2018/NR/C8NR00973B#!divAbstract
http://publications.jrc.ec.europa.eu/repository/handle/JRC109517
DOI: 10.1039/c8nr00973b
Type: Articles in periodicals and books
Abstract: Multi-functionalized nanoparticles are of great interest in biotechnology and biomedicine, especially for diagnostic and therapeutic purposes. However, at the moment the characterization of complex, multi-functional nanoparticles is still challenging and this hampers the development of advanced nanomaterials for biological applications. In this work, we have designed a model system consisting of gold nanoparticles functionalized with two differentially-terminated poly(ethylene oxide) ligands, providing both “stealth” properties and protein-binding capabilities to the nanoparticles. We use a combination of techniques (CLS, DLS, AF4, TEM, Z-potential, SDS-PAGE, and CD to: i) monitor and quantify the ratios of ligand molecules per nanoparticle; ii) determine the effect of coating density on nonspecific protein adsorption; iii) to assess the number and structure of the covalently-bound proteins. This article aims at comparing the complementary outcomes from typical and orthogonal techniques used on nanoparticle characterization by employing a versatile nanoparticle-ligands-biomolecule model system.
JRC Directorate:Health, Consumers and Reference Materials

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