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dc.contributor.authorOJEA JIMENEZ ISAACen_GB
dc.contributor.authorCAPOMACCIO ROBIN BRUNOen_GB
dc.contributor.authorOSORIO INESen_GB
dc.contributor.authorMĖHN DÓRAen_GB
dc.contributor.authorCECCONE GIACOMOen_GB
dc.contributor.authorHUSSAIN ROHANAHen_GB
dc.contributor.authorSILIGARDI GIULIANOen_GB
dc.contributor.authorCOLPO PASCALen_GB
dc.contributor.authorROSSI FRANCOISen_GB
dc.contributor.authorGILLILAND DOUGLASen_GB
dc.contributor.authorCALZOLAI LUIGIen_GB
dc.date.accessioned2018-07-14T00:23:20Z-
dc.date.available2018-07-12en_GB
dc.date.available2018-07-14T00:23:20Z-
dc.date.created2018-06-15en_GB
dc.date.issued2018en_GB
dc.date.submitted2017-12-05en_GB
dc.identifier.citationNANOSCALE vol. 10 no. 21 p. 10173-10181en_GB
dc.identifier.issn2040-3364en_GB
dc.identifier.urihttp://pubs.rsc.org/en/Content/ArticleLanding/2018/NR/C8NR00973B#!divAbstracten_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC109517-
dc.description.abstractMulti-functionalized nanoparticles are of great interest in biotechnology and biomedicine, especially for diagnostic and therapeutic purposes. However, at the moment the characterization of complex, multi-functional nanoparticles is still challenging and this hampers the development of advanced nanomaterials for biological applications. In this work, we have designed a model system consisting of gold nanoparticles functionalized with two differentially-terminated poly(ethylene oxide) ligands, providing both “stealth” properties and protein-binding capabilities to the nanoparticles. We use a combination of techniques (CLS, DLS, AF4, TEM, Z-potential, SDS-PAGE, and CD to: i) monitor and quantify the ratios of ligand molecules per nanoparticle; ii) determine the effect of coating density on nonspecific protein adsorption; iii) to assess the number and structure of the covalently-bound proteins. This article aims at comparing the complementary outcomes from typical and orthogonal techniques used on nanoparticle characterization by employing a versatile nanoparticle-ligands-biomolecule model system.en_GB
dc.description.sponsorshipJRC.F.2-Consumer Products Safetyen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherROYAL SOC CHEMISTRYen_GB
dc.relation.ispartofseriesJRC109517en_GB
dc.titleRational design of multi-functional gold nanoparticles with controlled biomolecule adsorption: a multi-method approach for in-depth characterizationen_GB
dc.typeArticles in periodicals and booksen_GB
dc.identifier.doi10.1039/c8nr00973ben_GB
JRC Directorate:Health, Consumers and Reference Materials

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