Physicochemical characterisation of gold, silica and silver nanoparticles in water and in serum-containing cell culture media
This report presents the results from a study organised under the coordination of JRC as part of a project aiming at the adaptation of the in vitro micronucleus test (Test Guideline 487) for the assessment of manufactured NMs. The aim of the first step of the project was to evaluate the physicochemical characterisation of selected representative nanomaterials (5 nm gold, 30 nm gold, 22 nm silica, 30 nm citrate and 30 nm PVP stabilised silver nanoparticles) in pure water and in different complete culture media.
The results of the study show that using a combination of different characterisation techniques is important to providing reliable information about the agglomeration behaviour of the tested nanoparticles in complete cell culture media (CCM). Most of the materials exhibited mild agglomeration in serum containing CCM. Only the PVP functionalised silver nanoparticles showed a size distribution change in all of the culture media that is so small that it could be attributed to solely protein adsorption without notable agglomeration. Silica nanoparticles were found to be the most sensitive to interaction with serum containing CCM, showing massive concentration and time dependent agglomeration strongly affected by the CCM composition. Extensive agglomeration might lead also to the accelerated sedimentation of the particles changing drastically the true, effective dose that the cells will receive under in vitro conditions1, 2. Thus, it has to be investigated in more detail and taken in account when designing in vitro experiments in the next phase of the project.
DREWES Carine;
OJEA JIMENEZ Isaac;
MĖHN Dóra;
COLPO Pascal;
GIORIA Sabrina;
BOGNI Alessia;
PONTI Jessica;
KINSNER-OVASKAINEN Agnieszka;
GILLILAND Douglas;
RIEGO SINTES Juan;
2018-02-07
Publications Office of the European Union
JRC110379
978-92-79-77705-9 (online),
978-92-79-77704-2 (print),
1831-9424 (online),
1018-5593 (print),
EUR 29054 EN,
OP KJ-NA-29054-EN-N (online),
OP KJ-NA-29054-EN-C (print),
https://publications.jrc.ec.europa.eu/repository/handle/JRC110379,
10.2760/818663 (online),
10.2760/58721 (print),
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