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dc.contributor.authorKREYLING WOLFGANGen_GB
dc.contributor.authorMÖLLER WINFRIEDen_GB
dc.contributor.authorHOLZWARTH UWEen_GB
dc.contributor.authorHIRN S.en_GB
dc.contributor.authorWENK A.en_GB
dc.contributor.authorSCHLEH C.en_GB
dc.contributor.authorSCHAFFLER M.en_GB
dc.contributor.authorHABERL NADINEen_GB
dc.contributor.authorGIBSON PETERen_GB
dc.contributor.authorSCHITTNY JOHANNESen_GB
dc.identifier.citationACS NANO vol. 12 no. 8 p. 7771-7790en_GB
dc.description.abstractThe increasing use of gold nanoparticles leads to a possible increase of exposure by inhalation. Therefore, we have studied the deposition patterns of inhaled 20 nm gold nanoparticles (AuNP) in 7−90 day old rats and their biokinetics in 60 day old ones. Wistar−Kyoto rats inhaled intratracheally 20 nm 195Au-radiolabeled AuNP by negative pressure ventilation over 2 h. Immediately afterward lungs were excised, inflated and microwave dried. AuNP deposition was analyzed by single-photon emission computed tomography, computed-tomography and autoradiography. Completely balanced, quantitative biodistributions in major organs and all body tissues and total excretion were analyzed from 1 h to 28 d after inhalation. Intratracheal inhalation caused AuNP deposition predominately in the caudal lungs, independent of age. About 30% AuNP were deposited on airway epithelia and rapidly cleared by mucociliary clearance. About 80% of AuNP deposited in alveoli was relocated from the epithelium into the interstitium within 24 h and was inaccessible to broncho-alveolar lavage. During interstitial long-term retention, re-entrainment within macrophages back onto the lung epithelium and to the larynx and gastrointestinal tract (GIT) dominated AuNP clearance (rate 0.03 d−1) In contrast, AuNP-translocation across the air−blood barrier was much smaller leading to persistent retention in secondary organs and tissues in the ranking order liver > soft issue > spleen > kidneys > skeleton > blood > uterus > heart > brain. The age-independent, inhomogeneous AuNP deposition was probably caused by the negative pressure ventilation. Long-term AuNP clearance was dominated by macrophage-mediated transport from the interstitium to the larynx and GIT. Translocation across the rat air−blood barrier appeared to be similar to that of humans for similar sized AuNP.en_GB
dc.description.sponsorshipJRC.F.2-Consumer Products Safetyen_GB
dc.publisherAMER CHEMICAL SOCen_GB
dc.titleAge-dependent rat lung deposition patterns of inhaled 20 nanometer gold nanoparticles and their quantitative biokinetics in adult rats.en_GB
dc.typeArticles in periodicals and booksen_GB
JRC Directorate:Health, Consumers and Reference Materials

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