Title: Nanostructured surface bioactive composite scaffold for filling of bone defects
Authors: CIOCCA LEONARDOLESCI ISIDORORAGAZZINI SARAGIORIA SABRINAVALSESIA ANDREAPARRILLI ANNAPAOLASPADARI ALESSANDRODOZZA BARBARAMORA PAOLOPIATTELLI ADRIANOIEZZI GIOVANNATARSITANO ACHILLEBALDISSARRA PAOLO
Citation: Biointerface Research in Applied Chemistry vol. 10 no. 2 p. 5038-5047
Publisher: AMG Transcend Association
Publication Year: 2020
JRC N°: JRC112382
ISSN: 2069-5837 (online)
URI: https://publications.jrc.ec.europa.eu/repository/handle/JRC112382
DOI: 10.33263/BRIAC102.038047
Type: Articles in periodicals and books
Abstract: The aim of this study was to investigate the chemical and physical surface properties of a hybrid nanohydroxyapatite/collagen/polycaprolactone (nHA/Coll/PCL) material, and to test its in vitro biocompatibility and in vivo osteointegration. Mineralized collagen fibers with nHA were admixed with PCL at a weight proportion of 50:50. The material was characterized by transmission X-ray diffraction (XRD), electron microscopy (TEM), atomic force microscopy (AFM), force spectroscopy, X-ray Photoemission Spectroscopy (XPS), and biocompatibility testing using human mesenchymal stem cells (MSCs), and Hepatocyte carcinoma (HePG2) and primary osteogenic sarcoma (SAOS-2) cells as complementary tests. In addition, the ability of this material to fill three-wall bony defects was tested in the mandible of a sheep. The material had confirmed the relative low crystallinity of the HA having a nano-sized dimension, which was composed of only oxygen, carbon, calcium and phosphorus, without no residual cytotoxic element. Human MSCs on the surface scaffold showed high metabolic activity and a high rate of viability. Biocompatibility complementary testing using HePG2 and SAOS-2 cells showed good metabolic activity, and the lactate dehydrogenase assay using HePG2 cells demonstrated no significant cytotoxicity. Histological analysis of the in-vivo experimentation showed osteointegration of the material and the absence of inflammatory cells at the bone–scaffold interface. Some areas showed bone-cell seeding and isolated agglomerates of bone cells were evident in the inner scaffold.
JRC Directorate:Health, Consumers and Reference Materials

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