Please use this identifier to cite or link to this item:
|Title:||Establishing a systematic framework to characterise in vitro methods for human hepatic metabolic clearance|
|Authors:||GOULIARMOU VARVARA; LOSTIA ALFONSO; COECKE SANDRA; BERNASCONI CAMILLA; BESSEMS JOSEPH; DORNE JEAN-LOU; FERGUSON STEPHEN; TESTAI EMANUELA; GUNDERT-REMY URSULA; HOUSTON BRIAN; MONSHOUWER MARIO; NONG ANDY; PELKONEN OLAVI; MORATH SIEGFRIED; WETMORE BARBARA; WORTH ANDREW; ZANELLI UGO; ZORZOLI MARIA CHIARA; WHELAN MAURICE|
|Citation:||TOXICOLOGY IN VITRO vol. 53 p. 233-244|
|Publisher:||PERGAMON-ELSEVIER SCIENCE LTD|
|Type:||Articles in periodicals and books|
|Abstract:||Hepatic metabolic clearance is one of the most important factors driving the overall kinetics of chemicals including substances used in various product categories such as pesticides, biocides, pharmaceuticals, and cosmetics. A large number of in vitro systems from purified isozymes and subcellular organelles to hepatocytes in simple cultures and in complex scaffold setups are available for measuring hepatic metabolic clearance for different applications. However, there is currently no approach for systematically characterising and comparing these in vitro methods in terms of their design, applicability and performance. To address this, existing knowledge in the field of in vitro human hepatic metabolic clearance methods was gathered and analysed in order to establish a framework to systematically characterise methods based on a set of relevant components. An analogous framework would be also applicable for non-human in vitro systems. The components are associated with the biological test systems used (e.g. subcellular or cells), the in vitro method (e.g. number of cells, test item solubility), related analytical techniques, data interpretation methods (based on substrate depletion/metabolite formation), and performance assessments (precision and accuracy of clearance measurements). To facilitate the regulatory acceptance of this class of methods, it is intended that the framework provide the basis of harmonisation work within the OECD.|
|JRC Directorate:||Health, Consumers and Reference Materials|
Files in This Item:
There are no files associated with this item.
Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.