Title: A high throughput imaging database of toxicological effects of nanomaterials tested on HepaRG cells
Authors: JOOSSENS ELISABETHMACKO PETERPALOSAARI TAINAGERLOFF KIRSTEN BRITTAOJEA JIMENEZ ISAACGILLILAND DOUGLASNOVAK JAROSLAVFORTANER TORRENT SALVADORGINESTE JEAN-MICHELROMER ISABELLABRIFFA SOPHIEVALSAMI-JONES EUGENIALYNCH ISEULTWHELAN MAURICE
Citation: SCIENTIFIC DATA vol. 6 no. 46 p. 1-10
Publisher: NATURE PUBLISHING GROUP
Publication Year: 2019
JRC N°: JRC114500
ISSN: 2052-4463 (online)
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC114500
DOI: 10.1038/s41597-019-0053-2
Type: Articles in periodicals and books
Abstract: The large amount of existing nanomaterials demands rapid and reliable methods for testing their potential toxicological effect on human health, preferably by means of relevant in vitro techniques in order to reduce testing on animals. Combining high throughput workflows with automated high content imaging techniques allows deriving much more information from cell-based assays than the typical readouts (i.e. one measurement per well) with optical plate-readers. We present here a dataset including data based on a maximum of 14 different read outs (including viable cell count, cell membrane permeability, apoptotic cell death, mitochondrial membrane potential and steatosis) of the human hepatoma HepaRG cell line treated with a large set of nanomaterials, coatings and supernatants at different concentrations. The database, given its size, can be utilized in the development of in silico hazard assessment and prediction tools or can be combined with toxicity results from other in vitro test systems.
JRC Directorate:Health, Consumers and Reference Materials

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