Anti-biofouling activity of Ranaspumin-2 bio-surfactant immobilized on catechol-functional PMMA thin layer prepared by atmospheric plasma deposition

Deposition of polymeric thin layers bearing reactive functional groups is a 1 promising solution to provide functionality on otherwise inert surfaces, for instance, for bioconjugation purposes. Atmospheric pressure plasma (AP plasma) deposition technology offers many advantages as fast deposition rates, low cost, low waste generation and suitability for coating various kind of material surfaces. In this work, the AP plasma assisted copolymerization of methyl methacrylate (MMA) with a vinyl derivative of L-DOPA is studied to deposit coatings with reactive catechol/quinone groups suitable for protein covalent immobilization. The effect of adding to the monomer mixture a chemical cross-linker, between 0 and 2 mol%, is also studied to prepare robust plasma PMMA-based layers in liquid physiological media. The layer prepared with 0.2 mol% of cross-linker shows the best balance between stability in saline buffered media and surface functionalization. Bioconjugation via grafting of Ranaspumin-2 recombinant, a natural occurring surfactant protein, is carried out in a single step after plasma deposition. Protein immobilization is corroborated by Quartz Crystal Microbalance with Dissipation (QCM-D) and Surface Plasmon Resonance (SPR) analyses and confirmed via Epicocconone staining, X-Ray Photoemission Spectroscopy (XPS) and Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) measurements and surface wettability characterizations. The bio-functionalized layers presented an enhanced activity against the adhesion of Human Serum Albumin (HSA) indicating the potential of grafting of Ranaspumin 2 bio-surfactant to produce anti-biofouling functional coatings.

CZUBA Urszula;  QUINTANA Robert;  LASSAUX Patricia;  BOMBERA Radoslaw Wiktor;  CECCONE Giacomo;  BAÑULS CISCAR Jorge;  MORENO-COURANJOU Maryline;  DETREMBLEUR Christophe;  CHOQUET Patrick; 

2019-06-03

ELSEVIER BV

JRC114878

0166-6622 (online),   

https://publications.jrc.ec.europa.eu/repository/handle/JRC114878,   

10.1016/j.colsurfb.2019.02.049 (online),