Characterization methods for studying protein adsorption on nano-polystyrene beads

This work is dealing with the use of polystyrene (PS) nanoparticles as substrates for bioanalytical specificinteractions. Different techniques were used for the accurate characterization of the PS nanoparticlesof 100 nm and 196 nm before coating them with a layer of antibodies against immunoglobulins of typeE (aIgE), giving to the particle a specific functionality. The formation of the aIgE adsorbed layer wasmonitored using centrifugal particle separation (CPS) and centrifugal field flow fractionation (CF3) exper-iments, which allowed to determine the size changes and the adsorbed mass. Particle sizes were alsomeasured with DLS, used both as stand-alone instrument and coupled to CF3 (CF3-DLS). The comple-mentary information obtained from the CPS and CF3-DLS measurements allowed the estimation of thedensity of the aIgE shell. The proteins immobilized at the surface fully retained their activity, as proven bythe reactions between the functionalized PS-aIgE particles and immunoglobulins of type E (IgE) dispersedin suspensions prepared on purpose.

CONTADO Catia;  MĖHN Dóra;  GILLILAND Douglas;  CALZOLAI Luigi; 

2019-12-17

ELSEVIER SCIENCE BV

JRC116384

0021-9673 (online),   

https://www.sciencedirect.com/science/article/pii/S0021967319307678?via%3Dihub,    https://publications.jrc.ec.europa.eu/repository/handle/JRC116384,   

10.1016/j.chroma.2019.460383 (online),