Title: In vitro RHE skin sensitisation assays: Applicability to challenging substances
Authors: MEHLING ANNETTEADRIAENS ELSCASATI SILVIAHUBESCH BRUNOIRIZAR AMAIAKLARIC MARTINALETASIOVA SILVIAMANOU IRENEMULLER BORIS P.ROGGEN ERWINVAN VLIET ERWINBASKETTER DAVID
Citation: REGULATORY TOXICOLOGY AND PHARMACOLOGY vol. 108 no. 104473 p. 1-8
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Publication Year: 2019
JRC N°: JRC118174
ISSN: 0273-2300 (online)
URI: https://publications.jrc.ec.europa.eu/repository/handle/JRC118174
DOI: 10.1016/j.yrtph.2019.104473
Type: Articles in periodicals and books
Abstract: In the last 20 years, alternative approaches to the identification of skin sensitisation hazards have been at the forefront of the 3Rs and have helped refine the validation and acceptance processes. However, experience with the local lymph node assay showed that, post-validation, challenges still occurred, particularly when a wider diversity of chemical substances was addressed, a situation which will arise with validated in vitro alternatives. In the present work, a range of substances potentially challenging to assess in current nonanimal OECD test guidelines were evaluated in several of the emerging in vitro alternatives. Twelve such substances (of which just over half were known skin sensitisers) were assessed in 4 assays, all based on reconstructed human epidermis (RHE) models. For hazard identification, the overall predictive accuracy ranged around 70% for three assays, although for one (SensCeeTox), it fell below 50% when human data was used as the benchmark. In most cases, sensitivity was high, such that sensitisation was overpredicted. As the substances were challenging to assess in other nonanimal methods, the results indicate that the 3D RHE models may be a useful tool for assessing skin sensitisation potentials without needing to revert to animal use.
JRC Directorate:Health, Consumers and Reference Materials

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