Neuron-like cells generated from human umbilical cord lining-derived
mesenchymal stem cells as a new in vitro model for neuronal toxicity
screening: using magnetite nanoparticles as an example.
The wide employment of iron nanoparticles in environmental and occupational settings underlines their potential to enter the brain. Human cell-based systems are recommended as relevant models to reduce uncertainty and to improve prediction of human toxicity. This study aimed at demonstrating the in vitro differentiation of the human umbilical cord lining-derived-mesenchymal stem cells (hCL-MSCs) into neuron-like cells (hNLCs) and the benefit of using them as an ideal primary cell source of human origin for the neuronal toxicity of Fe3O4NPs (magnetite-nanoparticles). Neuron-like phenotype was confirmed by: live morphology; Nissl body staining; protein expression
of different neuronal-specific markers (immunofluorescent staining), at different maturation stages (i.e., day-3-early and day-8-full differentiated), namely B-tubulin III, MAP-2, enolase (NSE), glial protein, and almost no nestin and SOX-2 expression. Synaptic makers (SYN, GAP43, and PSD95) were also expressed. Fe3O4NPs determined a concentration- and time-dependent reduction of hNLCs
viability (by ATP and the Trypan Blue test). Cell density decreased (20–50%) and apoptotic effects were detected at 10 ug/mL in both types of differentiated hNLCs. Three-day-differentiated hNLCs were more susceptible (toxicity appeared early and lasted for up to 48 h) than 8-day-differentiated cells (delayed effects). The study demonstrated that (i) hCL-MSCs easily differentiated into neuronal-like
cells; (ii) the hNCLs susceptibility to Fe3O4NPs; and (iii) human primary cultures of neurons are new in vitro model for NP evaluation
DE SIMONE Uliana;
SPINILLO Arsenio;
CALONI Francesca;
GRIBALDO Laura;
COCCINI Teresa;
2021-07-07
MDPI
JRC118875
1422-0067 (online),
https://www.mdpi.com/1422-0067/21/1/271,
https://publications.jrc.ec.europa.eu/repository/handle/JRC118875,
10.3390/ijms21010271 (online),
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