The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

Digital PCR (dPCR) has developed considerably since the publication of the dMIQE guidelines in 2013, with advances in instrumentation, software, applications and our understanding of its technological potential. This has enabled direct application of measurements to rare genetic variant detection, pathogen quantification as well as improvement of quality in other applications such as massively parallel sequencing. It is also increasingly clear that dPCR is capable of unprecedented accuracy, which in some cases can offer International System of Units (SI) traceable nucleic acid measurement. As there are now thousands of laboratories with dPCR instruments globally, the potential for the method to impact on molecular research is wide ranging. Yet these developments also have associated challenges; data analysis steps including threshold setting can be difficult and pre-analytical steps required to purify, concentrate and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR we present an update to dMIQE, dMIQE2020, building on the original guidelines and considering some of the new challenges identified in the last seven years. We also present a new simplified dMIQE table format to assist researchers in providing key experimental information and to aid reviewers, editors and the wider scientific community in understanding the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology.

HUGGETT Jim;  WHALE Alexandra S.;  DE SPIEGELAERE Ward;  TRYPSTEEN Wim;  NOUR Afif Abdel;  BAE Young-Kyung;  BENES Vladimir;  BURKE Daniel;  CLEVELAND Megan;  CORBISIER Philippe;  DEVONSHIRE A.;  DONG Lianhua;  DRANDI Daniela;  FOY C.A.;  GARSON Jeremy A.;  HE Hua-Jun;  HELLEMANS Jan;  KUBISTA Mikael;  LIEVENS Antoon;  MAKRIGIORGOS Mike;  MILAVEC Mojca;  MUELLER Reinhold D.;  NOLAN Tania;  O'SULLIVAN Denise M.;  PHAFFL Michael W.;  RÖDIGER Stefan;  ROMSOS Erica L.;  SHIPLEY Gregory L.;  TALY Valérie;  UNTERGASSER Andreas;  WITTWER Carl T.;  BUSTIN Stephan A.;  VANDESOMPELE Jo; 

2020-10-20

AMER ASSOC CLINICAL CHEMISTRY

JRC119955

0009-9147 (online),   

https://publications.jrc.ec.europa.eu/repository/handle/JRC119955,   

10.1093/clinchem/hvaa125 (online),