In vitro cytotoxicity and cellular uptake evaluation of gold, silica and silver nanoparticles in five different cell lines:
Caco-2, A549, CHO, V79 and TK6
The Joint Research Centre (JRC) is the science and knowledge service of the European Commission and provides scientific and technical support to a wide range of European Union policies. In particular, the use of manufactured nanomaterials (NMs) that are increasingly used in consumer and health products raised concerns regarding potential unintended risks to humans and the environment. Under the umbrella of the Organisation for Economic Co-operation and Development (OECD) Working Party on Manufactured Nanoparticles (WPMN), the JRC has contributed to the development, optimisation and harmonisation of a number of test methods incl in vitro test methods suitable for risk assessment of NMs.
The present report is part of an OCED project that focuses on availability of vitro methods for the assessment of the genotoxic potential of nanomaterials. An international partnership incl. the JRC aims to adapt the in vitro micronucleus test (OECD Test Guideline 487) to specific testing requirements of nanomaterials in a stepwise approach. Within the first phase of the project, the physical-chemical properties of selected nanomaterials have been intensively characterised. This second report is now focussing on the evaluation of in vitro cytotoxicity testing and the uptake of selected representative nanomaterials. 5 nm gold, 30 nm gold, 22 nm silica, 30 nm citrate and 30 nm PVP stabilised silver nanoparticles have been analysed in 5 different cell lines.
BOGNI Alessia;
PONTI Jessica;
DREWES Carine;
KINSNER-OVASKAINEN Agnieszka;
BREMER Susanne;
2022-05-12
Publications Office of the European Union
JRC120791
978-92-76-44690-3 (online),
1831-9424 (online),
EUR 30908 EN,
OP KJ-NA-30908-EN-N (online),
https://publications.jrc.ec.europa.eu/repository/handle/JRC120791,
10.2760/959826 (online),
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