Physicochemical Characterization Cascade of Nanoadjuvant–Antigen Systems for Improving Vaccines
Adjuvants have been used for decades to enhance the immune response to vaccines, in particular for the subunit-based ones. Physicochemical properties of the adjuvant-protein antigen complexes, such as size, morphology, protein structure and binding, influence the overall efficacy and safety of the vaccine. Here we show how to perform an accurate physicochemical characterization of the NanoAlum-Ovalbumin complex. Using a combination of existing techniques, we developed a multi-staged characterization strategy based on measurements of increased complexity. This characterization cascade has the advantage of being very flexible and easily adaptable to any adjuvant-protein antigen combinations. It will contribute to control the quality of antigen-adjuvant complexes and immunological outcomes, ultimately leading to improved vaccines.
GUERRINI Giuditta;
VIVI Antonio;
GIORIA Sabrina;
PONTI Jessica;
MAGRI Davide;
HOEVELER Arnd;
MEDAGLINI Donata;
CALZOLAI Luigi;
2021-11-16
MDPI
JRC123967
2076-393X (online),
https://www.mdpi.com/2076-393X/9/6/544,
https://publications.jrc.ec.europa.eu/repository/handle/JRC123967,
10.3390/vaccines9060544 (online),
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