CERTIFICATION REPORT:
The certification of different mass fractions of GMB151 in soya bean powder:
Certified Reference Materials ERM®-BF443a, ERM®-BF443b, ERM®-BF443c, ERM® BF443d and ERM®-BF443e
This report describes the production of ERM®-BF443, which are soya bean powder materials certified for the mass fraction of GMB151 (unique identifier BCS-GM151-6). These materials were produced and certified in accordance with ISO 17034:2016 and ISO Guide 35:2017. ERM BF443 was produced within the scope of ISO 17034 accreditation. Genetically modified (GM) soya bean seeds of the GMB151 event and seeds from a non-GM soya bean variety were milled to obtain GM and non-GM seed powders with a similar particle size distribution. Mixtures of non-GM and GM soya bean seed powder were prepared gravimetrically. The CRMs are available in glass vials containing at least 1 g of dried soya bean powder, which were sealed under an atmosphere of argon. Between-unit homogeneity was quantified and stability during dispatch and storage was assessed in accordance with ISO Guide 35:2017. The minimum sample size for one measurement is 200 mg. The certified value was obtained from the gravimetric preparations, taking into account the genetic purity of the base materials with respect to the GMB151 soya bean and their water mass fractions. The certified values were confirmed by event-specific real-time PCR (measurements produced by a laboratory with demonstrated competence and adhering to ISO/IEC 17025:2017. Uncertainties of the certified values were calculated in accordance with ISO 17034:2016 and ISO Guide 35:2017 and include uncertainties related to possible inhomogeneity, instability and characterisation. The materials are intended for the calibration or quality control of quantitative PCR measurements to identify GMB151 soya bean and quantify its mass fraction. As with any reference material, they can be used for establishing control charts or validation studies.
KORTEKAAS Anna Maria;
SEGHERS John;
LEYS Hanne;
CONTRERAS LOPEZ Maria Concepcion;
TRAPMANN Stefanie;
2021-04-06
Publications Office of the European Union
JRC124589
978-92-76-32435-5 (online),
1831-9424 (online),
EUR 30647 EN,
OP KJ-NA-30647-EN-N (online),
https://publications.jrc.ec.europa.eu/repository/handle/JRC124589,
10.2760/539645 (online),
Additional supporting files
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